In Cars 2, Lightning McQueen and Mater approach the Colossus while they are playing. They honk their horns to tip him, causing him to tip backwards and emit a burst of smoke from his exhaust pipe, blowing Mater and McQueen away.
Mater: "Heh, heh." Lightning McQueen: "Wow. Yeah, uh. I don't know. Do you think...?" Mater: "This is gonna be good!" (Mater honks his horn and Lightning revs his engine. Colossus XXL tips over and hits the ground hard) Mater: "Ah, ha, ha, ha, ha, ha." Lightning McQueen: "Ha, ha, did you see that?" (they stop laughing when they hear oil from the exhaust) Mater: "Uh, oh. This ain't gonna be good." (Colossus XXL backfires a burst of smoke from his exhaust sending Lightning McQueen and Mater away)
—Lightning McQueen and Mater tip Colossus XXL.
It is unknown what animal he seems to represent (as making a moo similar to the tractors (cows)), as there is no such thing as a giant animal.