Team Relay #1 follows the same path as the previous event, Radiator Springs Circuit, but instead of playing as one character throughout the whole event, the player will have three different cars, changing each lap. In story mode, the characters are Lightning, Doc, and Mater, and the opposing teams are Luigi, Giovanni, and Yuri and Barry, Mia and Tia.
In order to tag the next car, the player must drive directly behind them and drive into them. The player's next car is made clear by a large yellow arrow that points down at the vehicle. If the player misses the car, they will have their speed dropped to zero.